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Phases | Inertsil HILIC

Inertsil HILIC - Hydrophilic Interaction Chromatography Columns      Unique Selectivity and Elution Order for Polar Compounds    Polar Compounds show Increased Retention with Increased Organic Content    Greatly reduced ion suppression in LCMS    Vast database of chromatographic applications available to assist in method development    Very low operating back pressure             What is HILIC mode? Hydrophilic Interaction Chromatography Analysis of highly polar and basic compounds has been problematic since the beginning of HPLC.  Some polar compounds are so difficult to analyze that they require high aqueous buffer content, even on the most inert reverse phase HPLC columns, resulting in imperfect peak shape and impaired MS sensitivity. HILIC was developed as an alternative to reverse phase chromatography for polar compounds, and has also shown great promise in SFC.  The elution order in HILIC mode is typically the opposite of what it might be in reverse phase mode. In HILIC, the HIGHER the organic concentration, the GREATER the retention of more polar compounds.   Advantages of HILIC mode High organic content mobile phases result in low operating back pressure and ability to run at high flow rates A high organic solvent concentration of the mobile phase will lead to a high sensitivity LC/MS analysis. Reduced Ion suppression effect in LC/MS analyses. More precise quantitation of polar compounds owing to improved peak shape and the rapid elution of hydrophobic sample components.     INERTSIL HILIC Column Characterization Poster: Comparison of HILIC and Fluorinated Columns Using LC and SFC for the Separation of Polar Basic Compounds in Drug Development   Particle Size Particle Shape Surface Area Pore Size Pore Volume Silica Purity Bonded Phase End- Capping Carbon Load pH Range USP Code 3, 5 µm Spherical 450 m2/g 100Å 1.05 mL/g 99.999% alkyl diol Yes 2% 2 - 7.5